NetworkBLAST

NetworkBLAST analyzes protein interaction networks across species to infer protein complexes that are conserved in evolution.
For comparing multiple networks use Networkblast-M; for an executable of NetworkBLAST please refer to here.

Set a new NetworkBLAST job

Select number of species: 2-species 1-species

Upload input data Species #1 PPI data Species #2 PPI data BLAST data for 2 species proteins Or select this to use example data: The example run compares the PPI networks of yeast and fly. The output consists of putative protein complexes that are conserved across the two networks.

Set algorithm parameters Density of a complex [0.5-0.99] BLAST threshold [1e-64..1e-7] False negatives #1 [0.0-0.8] False negatives #2 [0.0-0.8] Reliability computation threshold [150-5000]

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Authors

NetworkBLAST software was developed and written as a collaborative effort between Dr. Roded Sharan's laboratory at Tel Aviv University, Professor Richard Karp's laboratory at the University of California, Berkeley and Professor Trey Ideker's laboratory at the University of California, San Diego.
Web-server engine was developed by Maxim Kalaev at Dr. Roded Sharan's laboratory at TAU.

We'd like to thank also:

• Mike Smoot at UCSD - for DualLayout complexes vizulalization plugin code and a lot of assistance
• Ivan Ovcharenko at LLNL - for sharing CREME engine code
• The www.webweaver.nu site - for free cliparts
• Angus Turnbul - htmlhttprequest Ajax engine author

References

• Sharan, R., Suthram, S., Kelley, R.M., Kuhn, T., McCuine, S., Uetz, P., Sittler, T., Karp, R.M. and Ideker, T. (2005) Conserved patterns of protein interaction in multiple species. Proc Natl Acad Sci U S A, 102, 1974-1979.